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¹ Kırıkkale University, Veterinary Faculty, Animal nutrition and Nutritional Diseases Department, Kırıkkale, Turkey
² Kırıkkale University, Veterinary Faculty, Pharmacology and Toxicology Department, Kırıkkale, Turkey
³ Kırıkkale University, Veterinary Faculty, Physiology Department, Kırıkkale, Turkey
⁴ Kırıkkale University, Veterinary Faculty Pathology Department, Kırıkkale, Turkey - The aim of this study is to evaluate the effect of fluoride toxication on the contraction and histopathological changes of duodenum, jejunum and ileum of broiler chicken. A total of 80 Ross 308 one-day-old male chicks assigned to 2 treatment groups with 4 replicates of 10 birds per replicated. 1st group was control group and the second group was a fluoride toxicated group, which was given 800 mg / kg flouride with the diet. Between 42 and 49 days of the study, randomly 3 chickens were chosen from each replicate group. Therefore totally 24 chickens were used to determine the contractile activity and examine the histopathological changes in the intestine. The broilers were decapitated and the duodenum, jejunum and ileum preparations were isolated, and mounted under 1 g of basal tension at isolated organ bath. The acetylcholine contractions were examined. There were no significant difference in the contraction of jejunum and ileum preparations, but the duodenum contractions were significantly decreased in fluoride given group as compared to control group, and in fluoride given group 7 of 13 duodenal samples showed no response to ACh. Microscopically; duodenal villus height / villus width ratio was 3.88 in fluoride group, while 6.84 in the control group. This significant ratio difference showed that fluoride could have a toxic effect caused by the villi thickness with severe mononuclear cell infiltration, hyperplasia of the lymphoid follicles and mild haemorrhagia in lamina propria. However there were no histopathological changes in jejunum and ileum between fluoride and control groups. These histopathological results were supported by the pharmacological findings. It is concluded that fluoride toxication affected the duodenal contractions and caused histopathological changes on the normal villus structure of duodenum in the broiler chicken. Keywords : Fluoride toxication, small intestine, acetylcholine, histopathological changes