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¹ Selçuk Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, Kampüs, KONYA - This study evaluated the performance and the quality of egg and fatty acid profile of egg yolk from laying hens, fed with diets, containing different levels of soybean oil and acidulated sunflower oil soapstock. A total of 75 44-week-old Hy-Line W36 laying hens were distributed according to a completely randomized experimental design into five treatments, consisting of soybean oil, acidulated sunflower oil soapstock (ASS) dietary inclusion levels (25, 50, 75 and 100 %), with five replicates of three birds each. The experiment was lasted in 84 days. Dietary treatments had no significant effect on body weight change, egg production, feed intake, feed conversion ratio, egg weight and egg mass of laying hens. The inclusion of ASS in the diet of laying hens had no significant effect on egg specific gravity, egg shell breaking strength, egg shell weight and egg shell thickness, egg shape index, albumen index, yolk index, albumen pH, yolk pH but haugh unit L, a, b color value had significant effect. The inclusion of different levels of soybean oil and ASS in the diet of laying hens had no significant effect on palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acid contents of egg yolk. It is concluded that the soybean oil can be replacement by ASS without any adverse effect on performance and egg quality in laying hen diets. Keywords : Acidulated sunflower oil soapstock, egg quality, fatty acid, laying hens, performance, soybean oil